Jackass Number Two is the sequel to the 2002 comedy film, Jackass: The Movie. Most of the original cast came back to do the movie, including Johnny Knoxville, Bam Margera, Ryan Dunn, Chris Pontius, Steve-O, Dave England, Ehren McGhehey, Preston Lacy, and Jason "Wee-Man" Acuña. The only member not returning was Chris Raab ("Raab Himself"). Shooting began in January 2006. The release date for the film was September 22, 2006. The film also includes cameos by Luke Wilson, Mark Zupan (Murderball), Spike Jonze and cult film directors John Waters (A Dirty Shame), Jay Chandrasekhar (Super Troopers) and Mike Judge (Beavis and Butt-Head). Professional BMX rider Matt Hoffman and professional skater Tony Hawk both performed stunts. Rap group Three 6 Mafia also appears, as well as HIM singer Ville Valo, with Kat Von D.

Fans of the series have seen cast members shooting in various states in America but the Jackass cast refuse to say where they are filming as it may result in too many fans interfering with the filming process. They have also filmed in India, Australia, and Moscow. A few insights were leaked prior to the movie's release by Steve-O and Bam Margera via Radio Bam and Loveline.

On June 15, Yahoo! released the first official teaser for the movie. The stunts in the trailer included Knoxville riding a "rocket bike" off a ramp and a blindfolded Knoxville being rammed by a yak.

The official music single for the movie is "The Joker & The Thief", from Wolfmother's self-titled debut album. A music video was shot in Australia for the band and for the promotion of the movie. Also on the movie soundtrack is the song "Karazy", performed by Jackass star Chris Pontius and his band.

The film grossed $29 million during its opening weekend, taking the number one spot at the box office. The film grossed an additional $14,010,000 in its second week. As of October 1, 2006, the film had made $51,452,000, nearly as much as the previous film made in its entire run.

Just as the first TV series and first movie, Jackass: Number Two has essentially no plot, being instead a collection of stunts and pranks.

The film opens with the sound of The Ecstasy Of Gold from The Good, The Bad, And The Ugly score by Ennio Morricone and the cast members running through a cloud of dust in a slow western style, returning in the blaze of glory for what could be their final adventure. Behind them is revealed to be a group of large bulls chasing them through an abandoned neighborhood. The men, one by one, are taken down by the stampede, until only Johnny Knoxville and Bam Margera are left, and are chased through a house. Bam jumps through a window, and Johnny stops in his tracks to deliver his signature line, “Hi, I’m Johnny Knoxville, welcome to Jackass!”, and is pushed through a window by several bulls, and the title comes to screen, “Jackass: Number Two”.

The movie ends with a Busby Berkeley movie musical production number set to the Jerry Herman song "The Best Of Times", where the cast sing and dance while getting battered by violent stunts. One stunt involves Wee Man and Preston Lacy sliding onto a bunch of metal trash cans. Another involves Chris Pontius dancing in a flaming building and jumping safely onto a mat, at which point he is knocked off his feet by a fire hose. Another is Bam and Dunn dancing in cowboy outfits in front of a horse, Knoxville then spins around and slaps the horse, causing it to flee, pulling Bam and Dunn off their feet by a rope attached to their feet. This huge sequence might have been inspired by the fact that in the first film, a huge finale was planned in a similar fashion, but the contraption that was to be used for the stunt failed and could not be fixed in time. Also, as in the first film, Rip Taylor is seen at the end of the sequence.

Michael Medved gave Jackass Number Two zero stars (out of four) calling it a "..truly appalling.." film.